AA021
(Plasmid #215929)

• Purpose: Fragmid fragment: (guide cassette) guide expression; positive control cell surface
• Depositing Lab: John Doench
• Publication: McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi: 10.1016/j.xgen.2024.100519.

Backbone

• Vector backbone: pUC57-Kan
• Vector type: CRISPR ; Fragment

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: U6_v1; DR_v0 [EnAs]; sgCD4_v1 [EnAs]; DR_v1 [EnAs]; sgCD4_v2 [EnAs];
• gRNA/shRNA sequence: ACTTTGACATGTTCCCTGAGAGC; TGGGCTTACCACTGCTGTTCCCA
• Species: Enhanced Acidaminococcus sp.

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • AA021.gb
• A portion of this plasmid was derived from a plasmid made by: Genscript ID: U5664GC010-6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.10.25.564061v1 for bioRxiv preprint.

Information for Cloning Grade DNA (Catalog # 215929-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here