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Addgene

pYHY1
(Plasmid #215926)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215926 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYHY1
  • Backbone size (bp) 6431
  • Vector type
    Bacterial Expression, Synthetic Biology ; E. coli-Bacteroides shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Also contains erythromycin for selection in Bacteroides species
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYHY1 was a gift from Shannon Sirk (Addgene plasmid # 215926 ; http://n2t.net/addgene:215926 ; RRID:Addgene_215926)

  • For your References section:

    A molecular toolkit for heterologous protein secretion across Bacteroides species. Yeh YH, Kelly VW, Pour RR, Sirk SJ. bioRxiv. 2023 Dec 15:2023.12.14.571725. doi: 10.1101/2023.12.14.571725. Preprint. 10.1101/2023.12.14.571725 PubMed 38168418
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