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psiCHECK-1-BRAF_Mut1-SM
(Plasmid #215918)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215918 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut1
  • Species
    H. sapiens (human)
  • Mutation
    BRAF
  • Entrez Gene
    BRAF (a.k.a. B-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psiCHECK-1-BRAF_Mut1-SM was a gift from Kumiko Ui-Tei (Addgene plasmid # 215918 ; http://n2t.net/addgene:215918 ; RRID:Addgene_215918)

  • For your References section:

    Knockdown of 15-bp Deletion-Type v-raf Murine Sarcoma Viral Oncogene Homolog B1 mRNA in Pancreatic Ductal Adenocarcinoma Cells Repressed Cell Growth In Vitro and Tumor Volume In Vivo. Song J, Kobayashi Y, Asano Y, Sato A, Taniguchi H, Ui-Tei K. Cancers (Basel). 2022 Jun 28;14(13):3162. doi: 10.3390/cancers14133162. 10.3390/cancers14133162 PubMed 35804932
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