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pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D
(Plasmid #215910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TNRC6A
  • Species
    H. sapiens (human)
  • Mutation
    contain aa1224-1709, S1332D, S1346D, S1616D, S1691D
  • Entrez Gene
    TNRC6A (a.k.a. CAGH26, FAME6, GW1, GW182, TNRC6)
  • Tag / Fusion Protein
    • Flag-HA-SBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D was a gift from Kumiko Ui-Tei (Addgene plasmid # 215910 ; http://n2t.net/addgene:215910 ; RRID:Addgene_215910)

  • For your References section:

    Identification of Phosphorylated Amino Acids in Human TNRC6A C-Terminal Region and Their Effects on the Interaction with the CCR4-NOT Complex. Munakata F, Suzawa M, Ui-Tei K. Genes (Basel). 2021 Feb 13;12(2):271. doi: 10.3390/genes12020271. 10.3390/genes12020271 PubMed 33668648
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