pcDNA3-Myc3-Cul3 H2M/H5M
(Plasmid #21591)

• Depositing Lab: Yue Xiong
• Publication: Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5437
• Vector type: Mammalian Expression
• Selectable markers: G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: cullin 3
• Alt name: CUL3
• Species: H. sapiens (human)
• Insert Size (bp): 2263
• Mutation: Mutated residues H2M/H5M
• GenBank ID: NM_003590
• Entrez Gene: CUL3 (a.k.a. CUL-3, NEDAUS, PHA2E)
• Tag / Fusion Protein:
  • myc3 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

H2H5 mutant has aa52-55 mutated (LSFE to AAAA), Y125A and R128A