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Addgene

psiCHECK-1-miR-99a-WT
(Plasmid #215880)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215880 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    three tandem repeats of seed-matched (SM) sequence of miR-99a-WT
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIR99A (a.k.a. MIRN99A, mir-99a)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psiCHECK-1-miR-99a-WT was a gift from Kumiko Ui-Tei (Addgene plasmid # 215880 ; http://n2t.net/addgene:215880 ; RRID:Addgene_215880)

  • For your References section:

    A robust model for quantitative prediction of the silencing efficacy of wild-type and A-to-I edited miRNAs. Tian S, Terai G, Kobayashi Y, Kimura Y, Abe H, Asai K, Ui-Tei K. RNA Biol. 2020 Feb;17(2):264-280. doi: 10.1080/15476286.2019.1678364. Epub 2019 Nov 1. 10.1080/15476286.2019.1678364 PubMed 31601146
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