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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 21586 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEBG
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Backbone manufacturerBruce Mayer Lab
- Backbone size w/o insert (bp) 6100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTNF receptor-associated factor 2
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Alt nameTRAF2
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SpeciesH. sapiens (human)
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Entrez GeneTRAF2 (a.k.a. MGC:45012, RNF117, TRAP, TRAP3)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX 5' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEBG-TRAF2 (GST) was a gift from John Kyriakis (Addgene plasmid # 21586 ; http://n2t.net/addgene:21586 ; RRID:Addgene_21586) -
For your References section:
Direct activation of mitogen-activated protein kinase kinase kinase MEKK1 by the Ste20p homologue GCK and the adapter protein TRAF2. Chadee DN, Yuasa T, Kyriakis JM. Mol Cell Biol. 2002 Feb . 22(3):737-49. 10.1128/MCB.22.3.737-749.2002 PubMed 11784851
Map uploaded by the depositor.