pMT2-MEKK3-K392A
(Plasmid
#21585)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21585 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMT2
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Backbone manufacturerUnknown
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemitogen-activated protein kinase kinase kinase 3
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Alt nameMEKK3
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SpeciesH. sapiens (human)
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MutationLysine 392 to Arginine
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Entrez GeneMAP3K3 (a.k.a. MAPKKK3, MEKK3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pMT2-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
lysine mutant activity unknown
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMT2-MEKK3-K392A was a gift from John Kyriakis (Addgene plasmid # 21585 ; http://n2t.net/addgene:21585 ; RRID:Addgene_21585) -
For your References section:
Direct activation of mitogen-activated protein kinase kinase kinase MEKK1 by the Ste20p homologue GCK and the adapter protein TRAF2. Chadee DN, Yuasa T, Kyriakis JM. Mol Cell Biol. 2002 Feb . 22(3):737-49. 10.1128/MCB.22.3.737-749.2002 PubMed 11784851