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Addgene

pSuperCRISPR-sgRNA-RL69-WT(TTTT)
(Plasmid #215847)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215847 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSuper.retro.puro
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA-RL69 with TTTT stretch
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Other

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSuperCRISPR-sgRNA-RL69-WT(TTTT) was a gift from Kumiko Ui-Tei (Addgene plasmid # 215847 ; http://n2t.net/addgene:215847 ; RRID:Addgene_215847)

  • For your References section:

    Enhancement of single guide RNA transcription for efficient CRISPR/Cas-based genomic engineering. Ui-Tei K, Maruyama S, Nakano Y. Genome. 2017 Jun;60(6):537-545. doi: 10.1139/gen-2016-0127. Epub 2017 Jan 26. 10.1139/gen-2016-0127 PubMed 28177825
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