pcDNA3.1-FLAG/HA-p54
(Plasmid
#215844)
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PurposeExpression of FLAG/HA-p54
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215844 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1 (+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman p54
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-FLAG/HA-p54 was a gift from Kumiko Ui-Tei (Addgene plasmid # 215844 ; http://n2t.net/addgene:215844 ; RRID:Addgene_215844) -
For your References section:
Control of the localization and function of a miRNA silencing component TNRC6A by Argonaute protein. Nishi K, Takahashi T, Suzawa M, Miyakawa T, Nagasawa T, Ming Y, Tanokura M, Ui-Tei K. Nucleic Acids Res. 2015 Nov 16;43(20):9856-73. doi: 10.1093/nar/gkv1026. Epub 2015 Oct 7. 10.1093/nar/gkv1026 PubMed 26446993