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Addgene

pIRESneo-FLAG/HA-FL
(Plasmid #215839)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215839 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESneo
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLAG/HA tagged firefly luciferase (FL)
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESneo-FLAG/HA-FL was a gift from Kumiko Ui-Tei (Addgene plasmid # 215839 ; http://n2t.net/addgene:215839 ; RRID:Addgene_215839)

  • For your References section:

    Control of the localization and function of a miRNA silencing component TNRC6A by Argonaute protein. Nishi K, Takahashi T, Suzawa M, Miyakawa T, Nagasawa T, Ming Y, Tanokura M, Ui-Tei K. Nucleic Acids Res. 2015 Nov 16;43(20):9856-73. doi: 10.1093/nar/gkv1026. Epub 2015 Oct 7. 10.1093/nar/gkv1026 PubMed 26446993
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