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Addgene

SpCas9-CBE6b-IVT
(Plasmid #215834)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215834 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV with a BR322 origin
  • Total vector size (bp) 8857
  • Vector type
    in vitro Transcription Template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CBE6b-SpCas9-2xUGI
  • Insert Size (bp)
    5325

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpCas9-CBE6b-IVT was a gift from David Liu (Addgene plasmid # 215834 ; http://n2t.net/addgene:215834 ; RRID:Addgene_215834)

  • For your References section:

    Phage-assisted evolution of highly active cytosine base editors with enhanced selectivity and minimal sequence context preference. Zhang E, Neugebauer ME, Krasnow NA, Liu DR. Nat Commun. 2024 Feb 24;15(1):1697. doi: 10.1038/s41467-024-45969-7. 10.1038/s41467-024-45969-7 PubMed 38402281
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