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Addgene

EXT1- D164A-pLJC2-3XFLAG
(Plasmid #215828)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215828 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLJC2
  • Total vector size (bp) 9676
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Exostosin glycosyltransferase 1 Mutant
  • Alt name
    EXT1-D164A
  • Species
    H. sapiens (human)
  • Entrez Gene
    EXT1 (a.k.a. EXT, LGCR, LGS, TRPS2, TTV)
  • Tag / Fusion Protein
    • 3X Flag

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EXT1- D164A-pLJC2-3XFLAG was a gift from Yoav Shaul (Addgene plasmid # 215828 ; http://n2t.net/addgene:215828 ; RRID:Addgene_215828)
  • For your References section:

    The exostosin glycosyltransferase 1/STAT3 axis is a driver of breast cancer aggressiveness. Solaimuthu B, Khatib A, Tanna M, Karmi A, Hayashi A, Abu Rmaileh A, Lichtenstein M, Takoe S, Jolly MK, Shaul YD. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2316733121. doi: 10.1073/pnas.2316733121. Epub 2024 Jan 12. 10.1073/pnas.2316733121 PubMed 38215181