SpCas9-CBE6a
(Plasmid
#215819)
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PurposeExpress CBE6a (with SpCas9) in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215819 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV with a BR322 origin
- Total vector size (bp) 8778
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCBE6a-SpCas9-2xUGI
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Insert Size (bp)5325
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Entrez Genecas9 (a.k.a. SD90_RS03825, SD90_03830)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SpCas9-CBE6a was a gift from David Liu (Addgene plasmid # 215819 ; http://n2t.net/addgene:215819 ; RRID:Addgene_215819) -
For your References section:
Phage-assisted evolution of highly active cytosine base editors with enhanced selectivity and minimal sequence context preference. Zhang E, Neugebauer ME, Krasnow NA, Liu DR. Nat Commun. 2024 Feb 24;15(1):1697. doi: 10.1038/s41467-024-45969-7. 10.1038/s41467-024-45969-7 PubMed 38402281