EXT1-pLJC2-3XFLAG
(Plasmid
#215818)
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PurposeEctopic expression of EXT-1 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215818 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLJC2
- Total vector size (bp) 9676
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameExostosin glycosyltransferase 1
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Alt nameEXT1
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SpeciesH. sapiens (human)
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Insert Size (bp)2328
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Entrez GeneEXT1 (a.k.a. EXT, LGCR, LGS, TRPS2, TTV)
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Tags
/ Fusion Proteins
- 3X Flag
- 3X Flag
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EXT1-pLJC2-3XFLAG was a gift from Yoav Shaul (Addgene plasmid # 215818 ; http://n2t.net/addgene:215818 ; RRID:Addgene_215818) -
For your References section:
The exostosin glycosyltransferase 1/STAT3 axis is a driver of breast cancer aggressiveness. Solaimuthu B, Khatib A, Tanna M, Karmi A, Hayashi A, Abu Rmaileh A, Lichtenstein M, Takoe S, Jolly MK, Shaul YD. Proc Natl Acad Sci U S A. 2024 Jan 16;121(3):e2316733121. doi: 10.1073/pnas.2316733121. Epub 2024 Jan 12. 10.1073/pnas.2316733121 PubMed 38215181