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Addgene

pcDNA3.1-SARS-CoV-2-M
(Plasmid #215813)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215813 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5414
  • Total vector size (bp) 6083
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Membrane
  • Insert Size (bp)
    669
  • Entrez Gene
    M (a.k.a. GU280_gp05)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-SARS-CoV-2-M was a gift from Thomas Gallagher (Addgene plasmid # 215813 ; http://n2t.net/addgene:215813 ; RRID:Addgene_215813)

  • For your References section:

    Dynamics of SARS-CoV-2 Spike Proteins in Cell Entry: Control Elements in the Amino-Terminal Domains. Qing E, Kicmal T, Kumar B, Hawkins GM, Timm E, Perlman S, Gallagher T. mBio. 2021 Aug 31;12(4):e0159021. doi: 10.1128/mBio.01590-21. Epub 2021 Aug 3. 10.1128/mBio.01590-21 PubMed 34340537
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