pNIC-HIS6-GST-TEV-AVITAG-GG
(Plasmid
#215809)
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Purpose(Empty Backbone) Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 215809 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepNIC28a
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.02.13.579886 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNIC-HIS6-GST-TEV-AVITAG-GG was a gift from ASAP Discovery & Frank von Delft (Addgene plasmid # 215809 ; http://n2t.net/addgene:215809 ; RRID:Addgene_215809) -
For your References section:
A selection of Golden Gate vectors to simplify recombinant protein production in Escherichia coli. Fairhead M, Koekemoer L, Williams E, von Delft F. bioRxiv 2024.02.13.579886 10.1101/2024.02.13.579886
