pMT2-HA-MKK3
(Plasmid #21580)

• Depositing Lab: John Kyriakis
• Publication: Molnar et al J Biol Chem. 1997 May 16. 272(20):13229-35.

Backbone

• Vector backbone: pMT2
• Backbone manufacturer: Unknown
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mitogen-activated protein kinase kinase 3
• Alt name: MKK3
• Species: H. sapiens (human)
• Entrez Gene: MAP2K3 (a.k.a. MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2, SAPKK2)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pMT2-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is an amino acid change from A to T at position 100, as seen from Addgene sequencing. Author does not feel this will cause any problems, and is not near critical residues.