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Addgene

pBH504
(Plasmid #215782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    NBU1 (R6K/RP4)
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Selectable markers
    Tetracycline (Bacteroides)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LacI(YQR)
  • Insert Size (bp)
    1083
  • Promoter Bt1311

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    sgRNA-ribozyme cassette
  • Insert Size (bp)
    213
  • Promoter P(O1)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    dCas9
  • Insert Size (bp)
    4107
  • Promoter P1

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBH504 was a gift from Corey Wilson (Addgene plasmid # 215782 ; http://n2t.net/addgene:215782 ; RRID:Addgene_215782)

  • For your References section:

    Transcriptional programming in a Bacteroides consortium. Huang BD, Groseclose TM, Wilson CJ. Nat Commun. 2022 Jul 6;13(1):3901. doi: 10.1038/s41467-022-31614-8. 10.1038/s41467-022-31614-8 PubMed 35794179
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