Bmi1-overexpression
(Plasmid #21577)

• Depositing Lab: Sally Temple
• Publication: Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Backbone

• Vector backbone: FUGW
• Backbone manufacturer: D. Baltimore
• Backbone size w/o insert (bp): 9941
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stable2 bacteria
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Bmi-1
• Species: M. musculus (mouse)
• Insert Size (bp): 2500
• GenBank ID: NM_007552
• Entrez Gene: Bmi1 (a.k.a. Bmi-1, Pcgf4)
• Tag / Fusion Protein:
  • ires-eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (destroyed during cloning)
• 3′ cloning site: Not1 (partial digest) (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Bmi-1 cDNA cloned into pIRES-eGFP first. The Bmi1-ires-eGFP was then cut out and cloned into FUGW.