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pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated
(Plasmid #215769)

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Item Catalog # Description Quantity Price (USD)
Plasmid 215769 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 5311
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TRAC-5' homology arm
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    312
  • Entrez Gene
    TRAC (a.k.a. IMD7, TCRA, TRA, TRCA)

Gene/Insert 2

  • Gene/Insert name
    P2A-CD19.28.zeta-bgH
  • Species
    H. sapiens (human), M. musculus (mouse), B. taurus (bovine)
  • Insert Size (bp)
    2015
  • Entrez Gene
    CD19 (a.k.a. B4, CVID3)

Gene/Insert 3

  • Gene/Insert name
    TRAC-3' homology arm
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    300
  • Mutation
    Cas12a PAM mutated
  • Entrez Gene
    TRAC (a.k.a. IMD7, TCRA, TRA, TRCA)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    TRAC homology arms were sourced from Roth et al Nature 2018 (PMID: 29995861, see also Addgene ID Plasmid #112021) by the Alexander Marson Lab; one mutation was added to prevent binding of the AsCas12a nuclease

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated was a gift from Dimitrios Wagner (Addgene plasmid # 215769 ; http://n2t.net/addgene:215769 ; RRID:Addgene_215769)

  • For your References section:

    Combining different CRISPR nucleases for simultaneous knock-in and base editing prevents translocations in multiplex-edited CAR T cells. Glaser V, Flugel C, Kath J, Du W, Drosdek V, Franke C, Stein M, Pruss A, Schmueck-Henneresse M, Volk HD, Reinke P, Wagner DL. Genome Biol. 2023 Apr 24;24(1):89. doi: 10.1186/s13059-023-02928-7. 10.1186/s13059-023-02928-7 PubMed 37095570
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