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Addgene

Bmi1 shRNA1
(Plasmid #21576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUGW-H1
  • Backbone manufacturer
    Ivanova, NB
  • Backbone size w/o insert (bp) 10130
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stable2 bacteria
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bmi-1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    60
  • GenBank ID
    NM_007552
  • Entrez Gene
    Bmi1 (a.k.a. Bmi-1, Pcgf4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site Sma1 (destroyed during cloning)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid targets the open reading frame of BMI-1. The 19mer target sequence is: GAGATAATAAGCTTGTCTA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Bmi1 shRNA1 was a gift from Sally Temple (Addgene plasmid # 21576 ; http://n2t.net/addgene:21576 ; RRID:Addgene_21576)

  • For your References section:

    shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway in NSC self-renewal during development. Fasano CA, Dimos JT, Ivanova NB, Lowry N, Lemischka IR, Temple S. Cell Stem Cell. 2007 Jun 7. 1(1):87-99. 10.1016/j.stem.2007.04.001 PubMed 18371338
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