scFv-IFP2.0N
(Plasmid
#215746)
-
PurposeExpresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0
-
Depositing Lab
-
Sequence Information
-
Sequences (1) — Accept Affinity Reagent Sequence Policy
-
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 215746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepHR-scFv-GCN4
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameIFP2.0N
- Promoter SFFV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cttctgttcgcgcgcttc (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE was a gift from Ron Vale (Addgene plasmid #60906; RRID:Addgene_60906). IFP2.0-C1 was a gift from Michael Davidson & Xiaokun Shu (Addgene plasmid # 54784; RRID:Addgene_54784).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
scFv-IFP2.0N was a gift from Albert Jeltsch (Addgene plasmid # 215746 ; http://n2t.net/addgene:215746 ; RRID:Addgene_215746) -
For your References section:
Modular dual-color BiAD sensors for locus-specific readout of epigenome modifications in single cells. Kohler AR, Hausser J, Harsch A, Bernhardt S, Haussermann L, Brenner LM, Lungu C, Olayioye MA, Bashtrykov P, Jeltsch A. Cell Rep Methods. 2024 Mar 26:100739. doi: 10.1016/j.crmeth.2024.100739. 10.1016/j.crmeth.2024.100739 PubMed 38554702