pDual-IN-SMN1
(Plasmid
#215721)
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Purposedual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215721 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTomatoSlicer
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSMN1 EXON 7
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SpeciesH. sapiens (human)
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Entrez GeneSMN1 (a.k.a. BCD541, GEMIN1, SMA, SMA1, SMA2, SMA3, SMA4, SMA@, SMN, SMNT, T-BCD541, TDRD16A)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDual-IN-SMN1 was a gift from Chaolin Zhang (Addgene plasmid # 215721 ; http://n2t.net/addgene:215721 ; RRID:Addgene_215721) -
For your References section:
CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements. Recinos Y, Ustianenko D, Yeh YT, Wang X, Jacko M, Yesantharao LV, Wu Q, Zhang C. Nat Commun. 2024 May 7;15(1):3839. doi: 10.1038/s41467-024-47140-8. 10.1038/s41467-024-47140-8 PubMed 38714659