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Addgene

Ecoli PancACe
(Plasmid #215712)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215712 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 6400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PancACe
  • Alt name
    acetyl-CoA biosensor
  • Species
    E. coli, A. victoria
  • Insert Size (bp)
    1158
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cpGFP portion of the gene was cloned from Addgene plasmid #64854 (Jin Zhang).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.12.31.573774 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ecoli PancACe was a gift from Katharine Diehl (Addgene plasmid # 215712 ; http://n2t.net/addgene:215712 ; RRID:Addgene_215712)

  • For your References section:

    A genetically-encoded fluorescent biosensor for visualization of acetyl-CoA in live cells. Smith JJ, Valentino TR, Ablicki AH, Banerjee R, Colligan AR, Eckert DM, Desjardins GA, Diehl KL. bioRxiv 2023.12.31.573774 10.1101/2023.12.31.573774
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