Cytoplasmic cpGFP
(Plasmid #215709)

• Purpose: Expresses cpGFP in the cytoplasm
• Depositing Lab: Katharine Diehl
• Publication: Smith et al bioRxiv 2023.12.31.573774

Backbone

• Vector backbone: pLJM1
• Backbone size w/o insert (bp): 7300
• Total vector size (bp): 8200
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cpGFP
• Alt name: circularly permuted green fluorescent protein
• Species: A. victoria
• Insert Size (bp): 885
• Mutation: circularly permuted GFP
• Promoter: CMV
• Tags / Fusion Proteins:
  • cytoplasmic localization sequence (N terminal on insert)
  • FLAG (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The cpGFP gene was cloned from Addgene plasmid #64854 (Jin Zhang).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2023.12.31.573774 for bioRxiv preprint.