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Addgene

pMS59
(Plasmid #215680)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215680 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDD162
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
  • Alt name
    eft-3p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
  • gRNA/shRNA sequence
    GTTTGAGTAGAGCACTCAGG
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS59 was a gift from Patrick Phillips (Addgene plasmid # 215680 ; http://n2t.net/addgene:215680 ; RRID:Addgene_215680)

  • For your References section:

    Expansion of the split hygromycin toolkit for transgene insertion in Caenorhabditis elegans. Moerdyk-Schauwecker MJ, Jahahn EK, Munoz ZI, Robinson KJ, Phillips PC. MicroPubl Biol. 2024 Jan 29;2024:10.17912/micropub.biology.001091. doi: 10.17912/micropub.biology.001091. eCollection 2024. 10.17912/micropub.biology.001091 PubMed 38351905
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