pMS110
(Plasmid #215674)

• Purpose: Split hygromycinR landing pad insertion plasmid for ChrIII
• Depositing Lab: Patrick Phillips
• Publication: Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.17912/micropub.biology.001091. doi: 10.17912/micropub.biology.001091. eCollection 2024.

Backbone

• Vector backbone: pMS63
• Vector type: Worm Expression, Cre/Lox, CRISPR
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
• Species: C. elegans (nematode), Synthetic
• Mutation: 3' ∆HYGR is promoterless and encodes aa60-341

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pMS110.gb
• A portion of this plasmid was derived from a plasmid made by: SEC is from pDD282 (Addgene #66823); ∆HYGR coding sequence is from pCFJ1663 (Addgene #51484)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry