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Addgene

pMS110
(Plasmid #215674)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215674 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMS63
  • Vector type
    Worm Expression, Cre/Lox, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5'HA + synthetic guide site3 + 3'ΔHygR:unc-54 3' UTR + lox2272 + SEC + lox2272 + 3'HA
  • Species
    C. elegans (nematode), Synthetic
  • Mutation
    3' ∆HYGR is promoterless and encodes aa60-341

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    SEC is from pDD282 (Addgene #66823); ∆HYGR coding sequence is from pCFJ1663 (Addgene #51484)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS110 was a gift from Patrick Phillips (Addgene plasmid # 215674 ; http://n2t.net/addgene:215674 ; RRID:Addgene_215674)

  • For your References section:

    Expansion of the split hygromycin toolkit for transgene insertion in Caenorhabditis elegans. Moerdyk-Schauwecker MJ, Jahahn EK, Munoz ZI, Robinson KJ, Phillips PC. MicroPubl Biol. 2024 Jan 29;2024:10.17912/micropub.biology.001091. doi: 10.17912/micropub.biology.001091. eCollection 2024. 10.17912/micropub.biology.001091 PubMed 38351905
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