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Addgene

OPEN Beta2-microglobulin
(Plasmid #215657)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215657 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBR322
  • Backbone manufacturer
    New England Biolabs
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 5704
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    OPEN Beta2-microglobulin
  • Alt name
    OPEN-B2M
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    304
  • Mutation
    Histidine 31 to Cysteine
  • Entrez Gene
    B2M (a.k.a. IMD43)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7F
  • 3′ sequencing primer T7R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OPEN Beta2-microglobulin was a gift from Nikolaos Sgourakis (Addgene plasmid # 215657 ; http://n2t.net/addgene:215657 ; RRID:Addgene_215657)
  • For your References section:

    Universal open MHC-I molecules for rapid peptide loading and enhanced complex stability across HLA allotypes. Sun Y, Young MC, Woodward CH, Danon JN, Truong HV, Gupta S, Winters TJ, Font-Burgada J, Burslem GM, Sgourakis NG. Proc Natl Acad Sci U S A. 2023 Jun 20;120(25):e2304055120. doi: 10.1073/pnas.2304055120. Epub 2023 Jun 13. 10.1073/pnas.2304055120 PubMed 37310998