OPEN Beta2-microglobulin
(Plasmid #215657)

• Purpose: E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.
• Depositing Lab: Nikolaos Sgourakis
• Publication: Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;120(25):e2304055120. doi: 10.1073/pnas.2304055120. Epub 2023 Jun 13.

Backbone

• Vector backbone: pBR322
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 5704
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: OPEN Beta2-microglobulin
• Alt name: OPEN-B2M
• Species: H. sapiens (human)
• Insert Size (bp): 304
• Mutation: Histidine 31 to Cysteine
• Entrez Gene: B2M (a.k.a. IMD43)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry