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Addgene

pBTRCk-pVhb-maACR
(Plasmid #215651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBTRCk
  • Backbone size w/o insert (bp) 3217
  • Total vector size (bp) 6370
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    acyl-CoA reductase
  • Alt name
    fatty acyl-CoA/ACP reductase
  • Alt name
    ACR
  • Species
    Marinobacter aquaeolei VT8
  • Insert Size (bp)
    3153
  • GenBank ID
  • Promoter Vhb
  • Tag / Fusion Protein
    • Maltose binding protein and His tag (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTRCk-pVhb-maACR was a gift from Brian Pfleger (Addgene plasmid # 215651 ; http://n2t.net/addgene:215651 ; RRID:Addgene_215651)

  • For your References section:

    Anaerobic production of medium-chain fatty alcohols via a beta-reduction pathway. Mehrer CR, Incha MR, Politz MC, Pfleger BF. Metab Eng. 2018 Jul;48:63-71. doi: 10.1016/j.ymben.2018.05.011. Epub 2018 May 25. 10.1016/j.ymben.2018.05.011 PubMed 29807110
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