pACYC-pVhb-vfFadBA
(Plasmid
#215650)
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PurposepACYC Origin, CmR, with vfFadBA gene under Vhb promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC
- Backbone size w/o insert (bp) 4310
- Total vector size (bp) 7665
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namethiolase (FadA), β-hydroxy-acyl-CoA reductase (FadB)
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Alt nametrifunctional enzyme complex
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SpeciesAliivibrio fischeri ES114
- Promoter Vhb
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACYC-pVhb-vfFadBA was a gift from Brian Pfleger (Addgene plasmid # 215650 ; http://n2t.net/addgene:215650 ; RRID:Addgene_215650) -
For your References section:
Anaerobic production of medium-chain fatty alcohols via a beta-reduction pathway. Mehrer CR, Incha MR, Politz MC, Pfleger BF. Metab Eng. 2018 Jul;48:63-71. doi: 10.1016/j.ymben.2018.05.011. Epub 2018 May 25. 10.1016/j.ymben.2018.05.011 PubMed 29807110