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ptrc99a-pVhb-tdTER-fdh
(Plasmid #215649)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215649 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ptrc99a
  • Backbone size w/o insert (bp) 3093
  • Total vector size (bp) 5382
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    trans-enoyl-CoA reductase
  • Alt name
    TER
  • Species
    Treponema denticola
  • Insert Size (bp)
    1194
  • Promoter Vhb

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    formate dehydrogenase
  • Alt name
    Fdh
  • Species
    Candida boidinii
  • Insert Size (bp)
    1095
  • Promoter Vhb

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ptrc99a-pVhb-tdTER-fdh was a gift from Brian Pfleger (Addgene plasmid # 215649 ; http://n2t.net/addgene:215649 ; RRID:Addgene_215649)

  • For your References section:

    Anaerobic production of medium-chain fatty alcohols via a beta-reduction pathway. Mehrer CR, Incha MR, Politz MC, Pfleger BF. Metab Eng. 2018 Jul;48:63-71. doi: 10.1016/j.ymben.2018.05.011. Epub 2018 May 25. 10.1016/j.ymben.2018.05.011 PubMed 29807110
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