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Addgene

PAS_HeSAHS4
(Plasmid #215648)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215648 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDB179
  • Backbone manufacturer
    Twist bioscience (shared by Chris Bahl Group)
  • Backbone size w/o insert (bp) 5548
  • Total vector size (bp) 6019
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SAHS4
  • Alt name
    Secretory-abundant heat soluble protein 4
  • Species
    Hypsibius exemplaris (Freshwater tardigrade)
  • Insert Size (bp)
    471
  • Tag / Fusion Protein
    • SUMO (N terminal on backbone)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Backbone sequence was donated by Chris Bahl's group. Synthesis with insert was done by Twist Biosciences.
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PAS_HeSAHS4 was a gift from Pamela Silver (Addgene plasmid # 215648 ; http://n2t.net/addgene:215648 ; RRID:Addgene_215648)