PAS_HeSAHS4
(Plasmid
#215648)
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PurposeExpresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215648 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDB179
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Backbone manufacturerTwist bioscience (shared by Chris Bahl Group)
- Backbone size w/o insert (bp) 5548
- Total vector size (bp) 6019
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSAHS4
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Alt nameSecretory-abundant heat soluble protein 4
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SpeciesHypsibius exemplaris (Freshwater tardigrade)
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Insert Size (bp)471
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Tag
/ Fusion Protein
- SUMO (N terminal on backbone)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byBackbone sequence was donated by Chris Bahl's group. Synthesis with insert was done by Twist Biosciences.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PAS_HeSAHS4 was a gift from Pamela Silver (Addgene plasmid # 215648 ; http://n2t.net/addgene:215648 ; RRID:Addgene_215648)