PAS_RvSAHS4
(Plasmid #215646)

• Purpose: Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli
• Depositing Lab: Pamela Silver
• Publication: Bacterial expression plasmids for production of recombinant SUMO-tagged tardigrade SAHS proteins (unpublished)

Backbone

• Vector backbone: pCDB179
• Backbone manufacturer: Twist bioscience (shared by Chris Bahl Group)
• Backbone size w/o insert (bp): 5548
• Total vector size (bp): 6010
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SAHS4
• Alt name: Secretory-abundant heat soluble protein 4
• Species: Ramazzottius varieornatus (Water bear) (Tardigrade)
• Insert Size (bp): 462
• Tag / Fusion Protein:
  • SUMO (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • PAS_RvSAHS4.gb
• A portion of this plasmid was derived from a plasmid made by: Backbone sequence was donated by Chris Bahl's group. Synthesis with insert was done by Twist Biosciences.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry