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Purpose(Empty Backbone) 3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Zeo
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 21562 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size (bp) 6909
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Vector typeMammalian Expression, Lentiviral, RNAi
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Bleocin (Zeocin), 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionsDB3.1, 37oC, low salt LB
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNo insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti X2 Zeo DEST (749-3) was a gift from Eric Campeau (Addgene plasmid # 21562 ; http://n2t.net/addgene:21562 ; RRID:Addgene_21562) -
For your References section:
A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells. Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, Campisi J, Yaswen P, Cooper PK, Kaufman PD.. PLoS One. 2009 Aug 6;4(8):e6529. 10.1371/journal.pone.0006529 PubMed 19657394
Map uploaded by the depositor.
