OPEN HLA-A*02:01-BirA
(Plasmid #215569)

• Purpose: E. coli expression of BirA tagged HLA-A*02:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.
• Depositing Lab: Nikolaos Sgourakis
• Publication: Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;120(25):e2304055120. doi: 10.1073/pnas.2304055120. Epub 2023 Jun 13.

Backbone

• Vector backbone: pET24a(+)
• Backbone size w/o insert (bp): 5242
• Total vector size (bp): 6130
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: OPEN HLA-A*02:01 BirA
• Alt name: OPEN-A02
• Species: H. sapiens (human)
• Insert Size (bp): 888
• Mutation: Glycine 120 to Cysteine
• GenBank ID:
• Entrez Gene: HLA-A (a.k.a. HLAA)
• Promoter: T7
• Tag / Fusion Protein:
  • BirA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: T7F
• 3′ sequencing primer: T7R

Resource Information

• Supplemental Documents:
  • NGS_OPEN_A0201_BirA_pLann.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry