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Addgene

pMD1 anti-GFP nanobody-Tn5
(Plasmid #215567)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215567 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    3XFlag-pA-Tn5-Fl
  • Backbone size w/o insert (bp) 6755
  • Total vector size (bp) 8595
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    anti-GFP nanobody-Tn5 fusion
  • Species
    E. coli
  • Insert Size (bp)
    1840
  • Promoter T7
  • Tags / Fusion Proteins
    • 3xFlag (N terminal on insert)
    • intein-CBD (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer GATCAAGCTTAC
  • 3′ sequencing primer CAGAATTCGATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMD1 anti-GFP nanobody-Tn5 was a gift from Isabelle Mansuy (Addgene plasmid # 215567 ; http://n2t.net/addgene:215567 ; RRID:Addgene_215567)

  • For your References section:

    NanoTag - an IgG-free method for mapping DNA-protein interactions. Maria A. Dimitriu, Rodrigo G. Arzate-Mejía, Leonard C. Steg, Pierre-Luc Germain, Isabelle M. Mansuy. bioRxiv 2024.07.12.603224 10.1101/2024.07.12.603224
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