pMP11
(Plasmid #215559)

• Purpose: pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin
• Depositing Lab: Brian Pfleger
• Publication: Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.1016/j.ymben.2018.05.011. Epub 2018 May 25.

Backbone

• Vector backbone: pKD46
• Backbone size w/o insert (bp): 7991
• Total vector size (bp): 12095
• Vector type: Bacterial Expression, CRISPR, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Growth instructions: Temperature sensitive, plasmid can't replicate over 30C
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Cas9
• Species: Streptococcus pyogenes
• Insert Size (bp): 4104
• Promoter: J23105

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: gRNA targeting pBR322 origin of replication
• Species: Synthetic
• Promoter: tet

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pMP11.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry