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Addgene

Cyto-APEX2
(Plasmid #215552)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215552 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-Flag tag-APEX2-NES
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://pubmed.ncbi.nlm.nih.gov/37090610/ for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cyto-APEX2 was a gift from Nevan Krogan (Addgene plasmid # 215552 ; http://n2t.net/addgene:215552 ; RRID:Addgene_215552)

  • For your References section:

    An automated proximity proteomics pipeline for subcellular proteome and protein interaction mapping. Zhong X, Li Q, Polacco BJ, Patil T, DiBerto JF, Vartak R, Xu J, Marley A, Foussard H, Roth BL, Eckhardt M, Von Zastrow M, Krogan NJ, Huttenhain R. bioRxiv. 2023 Apr 12:2023.04.11.536358. doi: 10.1101/2023.04.11.536358. Preprint. 10.1101/2023.04.11.536358 PubMed 37090610
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