P2A-NLS-tdtomato-EPG backbone
(Plasmid
#215548)
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Purpose(Empty Backbone) An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 215548 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
P2A-NLS-tdtomato-EPG backbone was a gift from Kai Wang (Addgene plasmid # 215548 ; http://n2t.net/addgene:215548 ; RRID:Addgene_215548) -
For your References section:
Protocol for scarless genome editing of human pluripotent stem cell based on orthogonal selective reporters. Zhao Y, Pan Z, Hong Z, Sun M, Hong Y, Peng X, Li X, Wang X, Wang K. STAR Protoc. 2024 May 23;5(2):103084. doi: 10.1016/j.xpro.2024.103084. 10.1016/j.xpro.2024.103084 PubMed 38787727
