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Addgene

pPROEX-HTc-Flag3-Nrf2
(Plasmid #21553)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPROEX-HTc
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4779
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nuclear factor (erythroid-derived 2)-like 2
  • Alt name
    NFE2L2
  • Alt name
    NRF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1873
  • GenBank ID
    Q16236
  • Entrez Gene
    NFE2L2 (a.k.a. HEBP1, IMDDHH, NRF2, Nrf-2)
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • Flag3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site AvaI (not destroyed)
  • 5′ sequencing primer M13
  • 3′ sequencing primer pGEX
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPROEX-HTc-Flag3-Nrf2 was a gift from Yue Xiong (Addgene plasmid # 21553 ; http://n2t.net/addgene:21553 ; RRID:Addgene_21553)

  • For your References section:

    BTB protein Keap1 targets antioxidant transcription factor Nrf2 for ubiquitination by the Cullin 3-Roc1 ligase. Furukawa M, Xiong Y. Mol Cell Biol. 2005 Jan . 25(1):162-71. 10.1128/MCB.25.1.162-171.2005 PubMed 15601839
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