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pMRX-IP-hWIPI3-3×FLAG
(Plasmid #215511)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215511 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRXIPU
  • Backbone manufacturer
    Dr.Shoji Yamaoka of Tokyo Medical and Dental University
  • Backbone size w/o insert (bp) 6137
  • Total vector size (bp) 7238
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WD-repeat protein interacting with phosphoinositides 3
  • Alt name
    WIPI3
  • Alt name
    WD repeat domain 45B
  • Alt name
    WDR45B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1101
  • GenBank ID
    NM_019613.4
  • Entrez Gene
    WDR45B (a.k.a. NEDSBAS, WDR45L, WIPI-3, WIPI3)
  • Tag / Fusion Protein
    • 3×FLAG (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggatctgccATCGATataAAGCTTaaaGGATCCatgaacctcctgccg
  • 3′ sequencing primer GTCTTTGTAGTCgcctgaaccagagccGAATTCcagcttgtcatcggt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IP-hWIPI3-3×FLAG was a gift from Noboru Mizushima (Addgene plasmid # 215511 ; http://n2t.net/addgene:215511 ; RRID:Addgene_215511)

  • For your References section:

    Comprehensive analysis of autophagic functions of WIPI family proteins and their implications for the pathogenesis of beta-propeller associated neurodegeneration. Shimizu T, Tamura N, Nishimura T, Saito C, Yamamoto H, Mizushima N. Hum Mol Genet. 2023 Aug 7;32(16):2623-2637. doi: 10.1093/hmg/ddad096. 10.1093/hmg/ddad096 PubMed 37364041
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