pMRX-IP-muGFP-hATG2A mLIR
(Plasmid #215506)

• Purpose: Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.
• Depositing Lab: Noboru Mizushima
• Publication: Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-2637. doi: 10.1093/hmg/ddad096.

Backbone

• Vector backbone: pMRXIPU
• Backbone manufacturer: Dr.Shoji Yamaoka of Tokyo Medical and Dental University
• Backbone size w/o insert (bp): 6138
• Total vector size (bp): 12672
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Autophagy related 2A
• Alt name: ATG2A
• Species: H. sapiens (human)
• Insert Size (bp): 6534
• Mutation: P656R (natural variant with no functional relevance), LC3-interacting region (LIR, residues 1362–1365) is substituted with alanine.
• GenBank ID: NM_015104.3
• Entrez Gene: ATG2A (a.k.a. BLTP4A)
• Tag / Fusion Protein:
  • muGFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTGTACAAGggttcaggctcgGGATCCTCACGATGGCTGTGGCCATGG
• 3′ sequencing primer: CGGCCGCtTTACTCGAGacccGAATTCTCAGTCTTGGGCACTGTCCGA

Resource Information

• Supplemental Documents:
  • pMRX-IPU-muGFP-ATG2A mLIR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry