pDB5230
(Plasmid #215496)

• Purpose: A pAde6 backbone integrating plasmid expressing Pil1-mCherry-SpAtg8(1-115) under the inducible promoter 41nmt1
• Depositing Lab: Li-Lin Du
• Publication: Pil1 co-tethering assay to detect protein-protein interactions in the fission yeast Schizosaccharomyces pombe (unpublished)

Backbone

• Vector backbone: pAde6NotI
• Backbone size w/o insert (bp): 9317
• Total vector size (bp): 11084
• Vector type: Yeast Expression
• Selectable markers: Hygromycin ; ADE6

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Pil1-mCherry-SpAtg8(1-115)
• Species: S. pombe (fission yeast)
• Insert Size (bp): 2365
• Promoter: P41nmt1

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pDB5230-pAde6NotI-P41nmt1-Pil1-mCherry-Atg8(1-115)-hphMX.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry