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Addgene

pDB5228
(Plasmid #215495)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215495 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHis5NotI
  • Backbone size w/o insert (bp) 8060
  • Total vector size (bp) 9827
  • Vector type
    Yeast Expression
  • Selectable markers
    G418;HIS5

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pil1-mCherry
  • Species
    S. pombe (fission yeast)
  • Insert Size (bp)
    1767
  • Promoter P41nmt1

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDB5228 was a gift from Li-Lin Du (Addgene plasmid # 215495 ; http://n2t.net/addgene:215495 ; RRID:Addgene_215495)

  • For your References section:

    Pil1 Co-tethering Assay to Detect Protein-Protein Interactions in the Fission Yeast Schizosaccharomyces pombe. Pan ZQ, Yang YS, Du LL. Methods Mol Biol. 2025;2862:93-102. doi: 10.1007/978-1-0716-4168-2_7. 10.1007/978-1-0716-4168-2_7 PubMed 39527195
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