pDB5226
(Plasmid
#215493)
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PurposeA pAde6 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 215493 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAde6NotI
- Backbone size w/o insert (bp) 8719
- Total vector size (bp) 10486
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Vector typeYeast Expression
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Selectable markersHygromycin ; ADE6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePil1-mCherry
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SpeciesS. pombe (fission yeast)
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Insert Size (bp)1767
- Promoter P41nmt1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDB5226 was a gift from Li-Lin Du (Addgene plasmid # 215493 ; http://n2t.net/addgene:215493 ; RRID:Addgene_215493)
