pcDNA3-EGFP-C4-Nrf2
(Plasmid #21549)

• Depositing Lab: Yue Xiong
• Publication: Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6112
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418) ; G418

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nuclear factor (erythroid-derived 2)-like 2
• Alt name: NFE2L2
• Alt name: NRF2
• Species: H. sapiens (human)
• Insert Size (bp): 1825
• GenBank ID: Q16236
• Entrez Gene: NFE2L2 (a.k.a. HEBP1, IMDDHH, NRF2, Nrf-2)
• Tags / Fusion Proteins:
  • EGFP (N terminal on backbone)
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 27 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry