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Addgene

CAGGS-losSTOPlox-ClaI flp-in
(Plasmid #21548)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21548 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Bluescript
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression, Mouse Targeting
  • Selectable markers
    Neomycin (select with G418) ; hygromycin after flp-in

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Insert Size (bp)
    5400

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI or NheI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAGGS-losSTOPlox-ClaI flp-in was a gift from Rudolf Jaenisch (Addgene plasmid # 21548 ; http://n2t.net/addgene:21548 ; RRID:Addgene_21548)

  • For your References section:

    Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R. Genesis. 2006 Jan . 44(1):23-8. 10.1002/gene.20180 PubMed 16400644
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