Ph-NylC_pET28a
(Plasmid
#215425)
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PurposeExpression construct for Ph-NylC gene, codon optimized for expression in E. coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 215425 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePh-NylC
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Alt nameNylon oligomer hydrolase from Phytoactinopolyspora halotolerans
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SpeciesPhytoactinopolyspora halotolerans
Cloning Information
- Cloning method Gene Synthesis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ph-NylC_pET28a was a gift from Gregg Beckham (Addgene plasmid # 215425 ; http://n2t.net/addgene:215425 ; RRID:Addgene_215425) -
For your References section:
Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization. Bell EL, Rosetto G, Ingraham MA, Ramirez KJ, Lincoln C, Clarke RW, Gado JE, Lilly JL, Kucharzyk KH, Erickson E, Beckham GT. Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5. 10.1038/s41467-024-45523-5 PubMed 38336849