NylCp2_pET28a
(Plasmid
#215420)
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PurposeExpression construct for NylCp2 gene, codon optimized for expression in E. coli
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 215420 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNylCp2
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Alt nameNylon oligomer hydrolase from Kocuria sp.
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SpeciesKocuria sp.
Cloning Information
- Cloning method Gene Synthesis
Resource Information
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A portion of this plasmid was derived from a plasmid made byGene from nylon oligomer hydrolase described in Negoro, S., Kakudo, S., Urabe, I. & Okada, H. A new nylon oligomer degradation gene (nylC) on plasmid pOAD2 from a Flavobacterium sp. J. Bacteriol. 174, 7948-7953 (1992).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NylCp2_pET28a was a gift from Gregg Beckham (Addgene plasmid # 215420 ; http://n2t.net/addgene:215420 ; RRID:Addgene_215420) -
For your References section:
Natural diversity screening, assay development, and characterization of nylon-6 enzymatic depolymerization. Bell EL, Rosetto G, Ingraham MA, Ramirez KJ, Lincoln C, Clarke RW, Gado JE, Lilly JL, Kucharzyk KH, Erickson E, Beckham GT. Nat Commun. 2024 Feb 9;15(1):1217. doi: 10.1038/s41467-024-45523-5. 10.1038/s41467-024-45523-5 PubMed 38336849
